| D1PF | 28/05/2001 | Bolton Wanderers | 3-0 | Preston North End | Farrelly, Ricketts, Gardner (played at Millennium Stadium) | Highlights | 54,328 | Win |
| D1PSFL2 | 17/05/2001 | Bolton Wanderers | 3-0 | West Bromwich Albion | Bergsson, Gardner, Ricketts | | 23,515 | Win |
| D1PSFL1 | 13/05/2001 | West Bromwich Albion | 2-2 | Bolton Wanderers | Bergsson, Frandsen (pen) | | 18,167 | Draw |
| D1 | 06/05/2001 | Bolton Wanderers | 1-1 | Sheffield United | Holden D | | 14,836 | Draw |
| D1 | 01/05/2001 | Wolverhampton Wanderers | 0-2 | Bolton Wanderers | Holdsworth, Ricketts | | 16,242 | Win |
| D1 | 28/04/2001 | Barnsley | 0-1 | Bolton Wanderers | Ricketts | | 13,979 | Win |
| D1 | 21/04/2001 | Bolton Wanderers | 1-0 | Norwich City | Holdsworth | | 17,967 | Win |
| D1 | 18/04/2001 | Crewe Alexandra | 2-1 | Bolton Wanderers | Holdsworth | | 8,054 | Loss |
| D1 | 16/04/2001 | Crystal Palace | 0-2 | Bolton Wanderers | Marshall, Kolinko (og) | | 16,268 | Win |
| D1 | 13/04/2001 | Bolton Wanderers | 2-2 | Birmingham City | Bergsson, Holdsworth | | 15,025 | Draw |
| D1 | 03/04/2001 | Bolton Wanderers | 1-1 | Stockport County | Hendry | | 12,492 | Draw |
| D1 | 31/03/2001 | Bolton Wanderers | 2-2 | Wimbledon | Elliott R, Hendry | | 14,562 | Draw |
| D1 | 17/03/2001 | Nottingham Forest | 0-2 | Bolton Wanderers | Holdsworth, Farrelly | | 22,162 | Win |
| D1 | 10/03/2001 | Bolton Wanderers | 3-3 | Gillingham | Frandsen, Patterson (og), Holdsworth | | 13,161 | Draw |
| FAC5R | 07/03/2001 | Blackburn Rovers | 3-0 | Bolton Wanderers | | | 20,318 | Loss |
| D1 | 04/03/2001 | Fulham | 1-1 | Bolton Wanderers | Frandsen | | 16,468 | Draw |
| D1 | 23/02/2001 | Bolton Wanderers | 1-4 | Blackburn Rovers | Ricketts | | 20,017 | Loss |
| D1 | 20/02/2001 | Bolton Wanderers | 2-2 | Grimsby Town | Bergsson, Hansen | | 24,249 | Draw |
| FAC5 | 17/02/2001 | Bolton Wanderers | 1-1 | Blackburn Rovers | Ricketts | | 22,048 | Draw |
| D1 | 13/02/2001 | Portsmouth | 1-2 | Bolton Wanderers | Ricketts, Hansen | | 11,377 | Win |
| D1 | 10/02/2001 | Bolton Wanderers | 2-2 | Huddersfield Town | Bergsson, Frandsen | | 14,866 | Draw |
| D1 | 03/02/2001 | Queens Park Rangers | 1-1 | Bolton Wanderers | Frandsen | | 10,293 | Draw |
| FAC4 | 28/01/2001 | Bolton Wanderers | 5-1 | Scunthorpe United | Holdsworth 3, Nolan 2 | | 11,737 | Win |
| D1 | 20/01/2001 | Sheffield Wednesday | 0-3 | Bolton Wanderers | Gardner, Ricketts, Marshall | | 17,638 | Win |
| D1 | 13/01/2001 | Bolton Wanderers | 2-0 | Tranmere Rovers | Hansen, Hill (og) | | 15,493 | Win |
| FAC3 | 06/01/2001 | Bolton Wanderers | 2-1 | Yeovil Town | O'Kane, Ricketts | | 11,161 | Win |
| D1 | 01/01/2001 | Preston North End | 0-2 | Bolton Wanderers | Farrelly, Ricketts | | 15,863 | Win |
| D1 | 30/12/2000 | Bolton Wanderers | 0-1 | West Bromwich Albion | | | 18,985 | Loss |
| D1 | 26/12/2000 | Bolton Wanderers | 2-0 | Sheffield Wednesday | Holdsworth, Hendry | | 21,316 | Win |
| D1 | 23/12/2000 | Burnley | 0-2 | Bolton Wanderers | Ricketts 2 | | 19,552 | Win |
| D1 | 16/12/2000 | Wimbledon | 0-1 | Bolton Wanderers | Holdsworth | | 6,076 | Win |
| D1 | 09/12/2000 | Bolton Wanderers | 4-1 | Crewe Alexandra | Frandsen, Marshall, Bergsson, Nolan | | 12,836 | Win |
| D1 | 03/12/2000 | Bolton Wanderers | 2-1 | Watford | Gardner, Marshall | | 13,904 | Win |
| D1 | 25/11/2000 | Sheffield United | 1-0 | Bolton Wanderers | | | 14,962 | Loss |
| D1 | 18/11/2000 | Norwich City | 0-2 | Bolton Wanderers | Ricketts, Bergsson | | 15,224 | Win |
| D1 | 11/11/2000 | Bolton Wanderers | 2-0 | Barnsley | Ricketts, Gardner | | 13,406 | Win |
| D1 | 04/11/2000 | Birmingham City | 1-1 | Bolton Wanderers | Ricketts | | 20,043 | Draw |
| D1 | 31/10/2000 | Bolton Wanderers | 3-1 | Queens Park Rangers | Bergsson, Elliott R, Ricketts | | 10,180 | Win |
| D1 | 28/10/2000 | Bolton Wanderers | 3-3 | Crystal Palace | Bergsson, Ricketts, Frandsen | | 12,879 | Draw |
| D1 | 24/10/2000 | Watford | 1-0 | Bolton Wanderers | | | 11,799 | Loss |
| D1 | 21/10/2000 | Stockport County | 4-3 | Bolton Wanderers | Marshall 2, Ricketts | | 8,226 | Loss |
| D1 | 17/10/2000 | Bolton Wanderers | 0-0 | Nottingham Forest | | | 13,017 | Draw |
| D1 | 14/10/2000 | Bolton Wanderers | 2-1 | Wolverhampton Wanderers | Holdsworth, Bergsson | | 15,585 | Win |
| D1 | 06/10/2000 | Gillingham | 2-2 | Bolton Wanderers | Hansen, O'Kane | | 9,311 | Draw |
| D1 | 30/09/2000 | Bolton Wanderers | 0-2 | Fulham | | | 19,924 | Loss |
| D1 | 23/09/2000 | Blackburn Rovers | 1-1 | Bolton Wanderers | Hansen | | 23,660 | Draw |
| D1 | 16/09/2000 | Bolton Wanderers | 2-0 | Portsmouth | Holdsworth, Ricketts | | 14,113 | Win |
| D1 | 12/09/2000 | Grimsby Town | 0-1 | Bolton Wanderers | Ricketts | | 3,732 | Win |
| D1 | 09/09/2000 | Huddersfield Town | 2-3 | Bolton Wanderers | Holdsworth, Ricketts 2 | | 12,248 | Win |
| LC1L2 | 05/09/2000 | Macclesfield Town | 3-1 | Bolton Wanderers | Ricketts | | 2,235 | Loss |
| D1 | 28/08/2000 | Tranmere Rovers | 0-1 | Bolton Wanderers | Whitlow | | 9,350 | Win |
| D1 | 26/08/2000 | Bolton Wanderers | 2-0 | Preston North End | Rankin, Ricketts | | 19,954 | Win |
| LC1L1 | 22/08/2000 | Bolton Wanderers | 1-0 | Macclesfield Town | Holdsworth | | 4,957 | Win |
| D1 | 19/08/2000 | West Bromwich Albion | 0-2 | Bolton Wanderers | Rankin, Farrelly | | 17,316 | Win |
| D1 | 12/08/2000 | Bolton Wanderers | 1-1 | Burnley | Frandsen (pen) | | 20,662 | Draw |